DETERMINATION OF THE REPAIR PROPERTIES OF TAMAN OIL IN A MODEL OF FULL-LAYER STENCIL WOUNDS IN RATS
DOI:
https://doi.org/10.58407/bht.3.25.8Keywords:
Тamanu oil (Сalophyllum inophyllum L.), reparative effect, wound epithelializationAbstract
Purpose of the work. To investigate the effect of tamanu oil on the intensity of reparative processes in the skin after its mechanical damage, to analyze the speed of skin epithelialization processes in an in vivo model experiment under the conditions of tamanu oil exposure compared to the control and under the conditions of Bepanthen exposure.
Methodology. The modeling method was used (in vivo models of full-thickness stencil wounds on sexually mature rats, 3 groups of animals, one of which was a control, n=30), a chronic experiment lasting 14 days to study the reparative effect of tamanu oil in comparison with the control and the effect of another effective and more studied drug (bepanthen), during which observation, planimetric and histological study of reparative processes, as well as statistical research methods were conducted.
Scientific novelty. The effectiveness of the use of unfractionated tamanu oil on the processes of epithelialization of skin wounds after mechanical damage was analyzed; the intensity of reparative processes under the action of tamanu oil and bepanthen was compared, and the effectiveness of the action of tamanu oil on the course of morphological changes in tissues in the presence of a wound skin defect was assessed.
Conclusions. Tamanu oil accelerates proliferative processes in the wound, which is manifested in an earlier (2-3 days compared to the control) filling of the wound defect with granulation tissue and the formation of a soft scar. The replacement of granulations with coarse-fibrous connective tissue and epithelialization of the mechanical skin defect on day 14 under the influence of tamanu oil reached almost 82 % (16.3 % more effective compared to the control); in terms of effectiveness, it is only 3.8 % inferior to the reference drug (Bepanthen). In animals that received the study drug (Tamanu oil), more complete regenerative changes were observed, as well as an anti-inflammatory effect, as evidenced by the absence of neutrophils in the focus compared to the reference drug (Bepanthen), under the action of which in some areas a pronounced lymphoplasmacytic infiltration with admixtures of neutrophils is observed.
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