FREE RADICAL PROCESSES IN CARP TISSUES UNDER THE TOXIC INFLUENCE OF THE PREPARATION “KORAGEN” IN IN VITRO CONDITIONS
DOI:
https://doi.org/10.58407/bht.2.26.12Keywords:
lipid peroxidation in carp tissues, malondialdehyde, catalase activity, superoxide dismutase activity, antioxidant state factor, insecticide “Koragen”Abstract
Purpose of the work. Assessment of the redox status of carp tissues (Cyprinus carpio L.) under the action of toxic concentrations of the drug "Koragen" against the background of artificially induced oxidative stress.
Methodology. The experiment was conducted in vitro on isolated carp tissues (C. carpio) in the system of non-enzymatic lipid peroxidation. The choice of the degree of dilution of the drug was due to the search for its dangerous concentration for the redox homeostasis of the studied tissues under experimental conditions. The intensity of the course of oxidative processes was assessed by the accumulation of malondialdehyde, and the state of the enzymatic link of the antioxidant protection system - by superoxide dismutase and catalase activities. The direction of free radical processes towards the anti- or pro-oxidant component was assessed by calculating the antioxidant state factor. The content of total protein was determined photocolorimetrically according to O.N. Lowry. Statistical processing of the obtained data was carried out using generally accepted methods of mathematical statistics.
Scientific novelty. For the first time, the redox status of carp tissues (C. carpio) was assessed under the action of toxic concentrations of the drug “Koragen” against the background of artificially induced oxidative stress.
Conclusions. The features of the concentration and tissue-specific effect of the drug “Koragen” on the processes of lipid peroxidation and antioxidant defense enzymes in carp tissues under in vitro conditions were established. The ways of protecting humans and animals from the toxic effects of the drug in the areas of its agrochemical application were analyzed. Multidirectional changes in the studied tissues regarding the content of malondialdehyde were shown depending on the concentration of the drug and the time of its exposure in the tissues. Activation of superoxide dismutase activity in the liver, muscles and inhibition of this enzyme in erythrocytes were established in most variants of the experiment. The sensitivity of the catalase enzyme to the concentration of the drug and the exposure time was revealed.
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