CHANGES IN SELECTED LIPID METABOLISM PARAMETERS AND ALKALINE PHOSPHATASE ACTIVITY IN COMMON CARP (CYPRINUS CARPIO L.) UNDER COMBINED EXPOSURE TO HERBICIDES AND ZINC SALTS
DOI:
https://doi.org/10.58407/bht.1.26.6Keywords:
herbicides, zinc, common carp, total lipids, phospholipids, alkaline phosphatase, combined toxic effectsAbstract
One of the main pathways by which herbicides enter aquatic ecosystems is their intensive use in agriculture, which, in turn, may lead to interactions with other anthropogenic pollutants, particularly heavy metals. Therefore, the study of the combined effects of xenobiotics on aquatic organisms, as sensitive indicators of the ecotoxicological status of water bodies, is highly relevant, as such interactions may be reflected in changes in biochemical markers of metabolism.
Purpose of the work. was to assess the combined effects of the herbicides Zencor, Roundup, and 2,4-D and zinc salts on the content of total lipids, phospholipids, and alkaline phosphatase activity in the tissues of common carp (Cyprinus carpio L.).
Methodology. The study was conducted under laboratory experimental conditions using environmentally relevant concentrations of herbicides and zinc. The content of total lipids and phospholipids, as well as alkaline phosphatase activity, was determined in blood, muscle, gills, liver, and brain tissues using standard biochemical methods. Statistical processing of the obtained results was carried out by methods of variational statistics using the Statistica 10.0 software package. The assessment of the reliability of differences between the control and experimental groups was carried out using the Student's criterion (t-test).
Scientific novelty. of the study lies in the identification of tissue-specific features of changes in lipid metabolism and enzymatic activity in common carp under combined exposure to herbicides and zinc, as well as in revealing the modifying role of zinc in the manifestation of herbicide toxicity.
Conclusions. It was established that the combined exposure to herbicides and zinc leads to disturbances in lipid homeostasis, destabilization of membrane structures, and changes in alkaline phosphatase activity, which exhibit a pronounced tissue-specific pattern. The obtained results indicate the systemic nature of biochemical process disturbances and confirm the feasibility of using lipid metabolism indicators and alkaline phosphatase activity as biochemical markers of combined anthropogenic impact.
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