DEVELOPMENT OF A POWDER MIXTURE FOR PREPARING A DRINK WITH INGREDIENTS THAT PROMOTE INCREASE COGNITIVE ENDURANCE

Authors

DOI:

https://doi.org/10.58407/bht.2.25.7

Keywords:

cognitive endurance, polyphenols, microencapsulation, L-tyrosine, instant drink

Abstract

The aim of this work was to develop an instant powdered beverage enriched with microencapsulated polyphenols and neuro-supportive nutrients to enhance cognitive endurance under combat-related stress. The product is intended for use by military personnel operating in extreme environments that impair mental performance.

Methodology: The beverage formulation included epigallocatechin gallate (EGCG), L-tyrosine, caffeine, L-thea­nine, zinc citrate, and berry-derived anthocyanins. Polyphenols were microencapsulated using sodium alginate, gelatin, and maltodextrin, followed by freeze-drying. To reduce the bitterness of L-tyrosine and EGCG, a masking matrix based on maltodextrin and whey protein isolate was developed. The samples were analyzed for antioxidant activity (DPPH, FRAP), individual polyphenol content (HPLC), sensory attributes, solubility, and physical stability during 30-day storage.

Scientific novelty: This study presents an integrated approach combining microencapsulation, matrix masking, and functional formulation tailored to cognitive needs in high-stress settings. For the first time, a freeze-dried beverage matrix was designed to stabilize both EGCG and anthocyanins while simultaneously masking L-tyrosine bitterness without advanced encapsulation systems.

Conclusions: The developed beverage demonstrated high antioxidant activity, excellent solubility, and sensory acceptability. Microencapsulation significantly improved the retention of polyphenols and organoleptic quality. The formulation proved stable for at least 30 days at room temperature. The combination of ingredients was scientifically justified and technologically feasible. The product has potential for use in military nutrition as a convenient and effective means to support cognitive performance and endurance during prolonged stress.

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References

Ahles, S., Joris, P. J., & Plat, J. (2021). Effects of berry anthocyanins on cognitive performance, vascular function and cardiometabolic risk markers: A systematic review of randomized placebo-controlled intervention studies in humans. International Journal of Molecular Sciences, 22(12), 6482. https:// doi.org/10.3390/ijms22126482

Benzie, I. F. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Analytical Biochemistry, 239(1), 70–76. https://doi.org/ 10.1006/abio.1996.0292

Bińkowska, W., Szpicer, A., Stelmasiak, A., Wojtasik-Kalinowska, I., & Półtorak, A. (2024). Micro-encapsulation of polyphenols and their application in food technology. Applied Sciences, 14(24), 11954. https://doi.org/10.3390/app142411954

Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT – Food Science and Technology, 28(1), 25-30. https://doi.org/10.1016/ S0023-6438(95)80008-5

Deijen, J. B., Wientjes, C. J., Vullinghs, H. F., Cloin, P. A., & Langefeld, J. J. (1999). Tyrosine improves cognitive performance and reduces blood pressure in cadets after one week of a combat training course. Brain Research Bulletin, 48(2), 203-209. https://doi.org/10.1016/ s0361-9230(98)00163-4

Estevinho, B. N., & Rocha, F. (2022). Development of food–grade controlled delivery systems by microencapsulation of polyphenols with health benefits. In M. A. Grumezescu & A. M. Holban (Eds.), Handbook of functional beverages and human health (pp. 1089-1114). Springer. https://doi.org/ 10.1007/978-3-031-04797-8_42

Fan, J., & Smith, A. P. (2020). Effects of occupational fatigue on cognitive performance of staff from a train operating company: A field study. Frontiers in Psychology, 11, 558520. https://doi.org/ 10.3389/fpsyg.2020.558520

Fekete, M., Lehoczki, A., Tarantini, S., Fazekas Pongor, V., Csípő, T., Csizmadia, Z., & Varga, J. T. (2023). Improving cognitive function with nutritional supplements in aging: A comprehensive narrative review of clinical studies investigating the effects of vitamins, minerals, antioxidants, and other dietary supplements. Nutrients, 15(24), 5116. https://doi.org/10.3390/nu15245116

Gibson, E. L., & Green, M. W. (2002). Nutritional influences on cognitive function: Mechanisms of susceptibility. Nutrition Research Reviews, 15(1), 169-206. https://doi.org/10.1079/ NRR200131

Giles, G. E., Mahoney, C. R., Brunyé, T. T., Taylor, H. A., & Kanarek, R. B. (2017). Caffeine and theanine exert opposite effects on attention under emotional arousal. Canadian Journal of Physiology and Pharmacology, 95(1), 93-100. https://doi.org/10.1139/cjpp-2016-0498

Grabska Kobyłecka, I., Szpakowski, P., Król, A., Książek Winiarek, D., Kobyłecki, A., Głąbiński, A., & Nowak, D. (2023). Polyphenols and their impact on the prevention of neurodegenerative diseases and development. Nutrients, 15(15), 3454. https://doi.org/10.3390/nu15153454

International Organization for Standardization. (2005). Determination of substances characteristic of green and black tea – Part 2: Content of catechins in green tea – Method using high-performance liquid chromatography (ISO 14502-2:2005). https://www.iso.org/standard/ 31357.html

Islam, M. R., Rauf, A., Akter, S., Akter, H., Al Imran, M. I. K., Islam, S., Nessa, M., Shompa, C. J., Shuvo, M. N. R., Khan, I., Al Abdulmonem, W., Aljohani, A. S. M., Imran, M., & Iriti, M. (2025). Epigallo-catechin 3 gallate induced neuroprotection in neurodegenerative diseases: Molecular mechanisms and clinical insights. Molecular and Cellular Biochemistry, 480(6), 3363-3383. https://doi.org/10.1007/ s11010-025-05211-4

Kochubei, E., Lomovskiy, I., Lomovsky, O., Baktybekova, J., Koptev, V., Andreeva, A., Matveeva, A., Vorobyov, A., Tantawy, A. A., Eid, M. H., El Messery, T. M., & Bychkova, E. (2025). Optimization of microencapsulation of green tea extract for functional beverage development. Applied Food Research, 5(1), 100790. https://doi.org/10.1016/j.afres.2025.100790

Lieberman, H. R., Bathalon, G. P., Falco, C. M., Kramer, F. M., Morgan, C. A., & Niro, P. (2005). Severe decrements in cognition function and mood induced by sleep loss, heat, dehydration, and undernutrition during simulated combat. Biological Psychiatry, 57(4), 422-429. https://doi.org/ 10.1016/j.biopsych.2004.11.014

Mahoney, C. R., Castellani, J., Kramer, F. M., Young, A., & Lieberman, H. R. (2007). Tyrosine supple-mentation mitigates working memory decrements during cold exposure. Physiology & Behavior, 92(4), 575-582. https://doi.org/10.1016/j.physbeh.2007.05.003

Martin, K., Meeusen, R., Thompson, K. G., Keegan, R., & Rattray, B. (2018). Mental fatigue impairs endurance performance: A physiological explanation. Sports Medicine, 48(9), 2041-2051. https:// doi.org/10.1007/s40279-018-0946-9

McLellan, T. M., Caldwell, J. A., & Lieberman, H. R. (2016). A review of caffeine’s effects on cognitive, physical and occupational performance. Neuroscience & Biobehavioral Reviews, 71, 294-312. https://doi.org/10.1016/j.neubiorev.2016.09.001

Owen, G. N., Parnell, H., De Bruin, E. A., & Rycroft, J. A. (2008). The combined effects of L theanine and caffeine on cognitive performance and mood. Nutritional Neuroscience, 11(4), 193-198. https:// doi.org/10.1179/147683008X301513

Pervin, M., Unno, K., Ohishi, T., Tanabe, H., Miyoshi, N., & Nakamura, Y. (2018). Beneficial effects of green tea catechins on neurodegenerative diseases. Molecules, 23(6), 1297. https://doi.org/ 10.3390/molecules23061297

Wang, X., Wang, H., Zhang, F., Cui, Y., Zhang, D., & Shen, X. (2021). Threshold effects and interactive effects of total zinc and selenium intake on cognitive function in older adults. Clinical Nutrition ESPEN, 47, 383-389. https://doi.org/10.1016/j.clnesp.2021.11.001

Published

2025-07-10

How to Cite

Chernenko С. (2025). DEVELOPMENT OF A POWDER MIXTURE FOR PREPARING A DRINK WITH INGREDIENTS THAT PROMOTE INCREASE COGNITIVE ENDURANCE. Biota. Human. Technology, (2), 96–108. https://doi.org/10.58407/bht.2.25.7

Issue

Section

FOOD TECHNOLOGIES